Journal: Scientific Reports

Article Title: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

doi: 10.1038/s41598-018-36827-w

Figure Lengend Snippet: Binding specificity of 111 In-Z 08698 -X on BxPC-3 and DU145 cells. HER3 receptors in the blocked group were pre-saturated with excess of unlabeled Z 08698 . Data are presented as an average ± standard deviation from three cell culture dishes.

Article Snippet: HER3 expressing cell lines, BxPC-3 (pancreatic cancer) and DU145 (prostate cancer), were purchased from American Type Tissue Culture Collection (ATTC via LGC Promochem, Borås, Sweden) and cultured in RMPI-1640 media supplemented with 1% penicillin and 1% L-glutamine (all from Biochrom, Berlin, Germany) and 10% fetal bovine serum (Sigma-Aldrich, Germany) at 37 °C with 5% CO 2 .

Techniques: Binding Assay, Standard Deviation, Cell Culture

Journal: Scientific Reports

Article Title: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

doi: 10.1038/s41598-018-36827-w

Figure Lengend Snippet: Cellular processing of 111 In-Z 08698 -X on BxPC-3 (black) and DU145 cells (grey). Total cell bound activity (solid line) and internalized activity (dashed line) are displayed as percent of cell associated activity. The values were normalized to maximum cell-associated activity for each conjugate. Data are presented as an average ± standard deviation from three cell culture dishes.

Article Snippet: HER3 expressing cell lines, BxPC-3 (pancreatic cancer) and DU145 (prostate cancer), were purchased from American Type Tissue Culture Collection (ATTC via LGC Promochem, Borås, Sweden) and cultured in RMPI-1640 media supplemented with 1% penicillin and 1% L-glutamine (all from Biochrom, Berlin, Germany) and 10% fetal bovine serum (Sigma-Aldrich, Germany) at 37 °C with 5% CO 2 .

Techniques: Activity Assay, Standard Deviation, Cell Culture